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KMID : 0923620090090040133
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Immune Network
2009 Volume.9 No. 4 p.133 ~ p.137
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Further Characterization of Activin A-induced IgA Response in Murine B Lymphocytes
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Lee Hwa-Joung
Kim Pyeung-Hyeun
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Abstract
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We have recently shown that activin A, a member of TGF-β superfamily, stimulates mouse B cells to express IgA isotype but other isotypes. In the present study, we further characterized effects of activin A on B cell growth and IgA expression. We found that activin A did not have effect on LPS-stimulated cell viability. In parallel, CFSE staining analysis revealed that activin A did not alter cell division. An increase of IgA secretion by activin A was completely abrogated by anti-activin A Ab but not by anti-TGFβ1 Ab. In the same conditions, no other isotypes are significantly affected by each antibody treatment. Finally, activin A, as similar to TGF-β1, increased IgA secretion by mesenteric lymph node cells. These results suggest that activin A can specifically stimulate IgA response, independent of TGF-β in the gut.
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KEYWORD
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activin A, TGF-β, IgA, mesenteric lymph node, CFSE
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